Fig. 3.

Localization of 3C5, 4C3, 7D4 and 3B3(+) CS epitopes to vessels in the theca externa of large antral follicles and the ovarian medulla. (A) Section of a large antral follicle stained with H&E showing a large vessel (asterisk) at the boundary of the theca interna (ti) and theca externa, the membrana granulosa (g) and a small vessel in the theca externa (arrow). (B, C, D) Serial sections of the area shown in panel A. 3C5 (red, panel B), 4C3 (red, panel C) and 7D4 (red, panel D) localize to the vessels identified by staining with von Willebrand factor (green). The arrow indicates a small vessel in the theca externa and the asterisk a large blood vessel as also identified in panel A. (E) Section of a large antral follicle stained with H&E showing a large vessel (asterisk) in the theca externa. (F) Same section as panel E localizing 3B3(+) (red) and von Willebrand factor (green) around a large blood vessel (asterisk) as also identified in panel E. (G, H, I) Localization of LYVE-1 (red) and of 4C3 (green in G), 7D4 (green in H, I) in the theca externa of large antral follicles (G, H) and ovarian medulla (I). (J) Section of a similar region of the ovarian medulla to that shown in panel I stained with H&E and identifying a lymphatic vessel (asterisk) and an area with aterioles (triangle). (K) A large arteriole in the ovarian medulla stained with H&E. ( L) Serial section of the arteriole in panel K localizing 3B3(+) (red) to the muscularis layer and von Willebrand factor (green) identifying the endothelium. (M) 7D4 (green) localization to the muscularis (red, smooth muscle actin) of a large arteriole in the ovarian medulla. Bars = 20 μm.