Fig. 1.
Direct addition of stereoisomers CP-55,940 and CP-56,667 to naive spleen cell culture and their effect on the in vitro sRBC AFC response. Spleens from naive female B6C3F, mice were isolated aseptically and made into single-cell suspensions. The splenocytes were washed, adjusted to 1.0 × 107 cells/ml, and transferred in 500-µl aliquots to wells of a 48-wel culture plate. Quadruplicate cultures were prepared with vehicle [0.01% dimethylsulfoxide (DMSO) or 0.1% ethanol (EtOH) final concentration in culture], CP-55,940, or CP-56,667 and were sensitized with sRBC. Cultures were subsequently assayed, using sRBC, for their day 5 IgM antibody response by enumerating the number of AFC, spleen cell viability, and total recovered cells/culture. Bars, mean ± standard error as determined for each group. * p < 0.05, as determined by Dunnett’s t test, compared with the vehicle group.