Fig. 3.

Δ⁹-THC does not suppress PMA/Io-induced surface CD40L expression on CD4⁺ T cells. Splenocytes (5×10⁶ cells) were treated with various concentrations of Δ⁹-THC (5, 10, 15, and 20 μM) for 30 min and then stimulated with 40 nM PMA plus 0.5 μM ionomycin for 8 h. a Cell surface CD40L expression on activated CD4⁺ was determined by flow cytometry. Number in parentheses represents the percentage of CD4⁺CD40L⁺ cell population±SEM. b Bar graph is a representation of data in a. The * or ** indicates significant effect compared to vehicle control (VH=0.1 % EtOH) at p≤0.05 or p≤0.01, respectively. Results are representative of two separate experiments with three replicates per treatment group