Fig. 7.

GRE luciferase reporter activity in HEK293T cells treated with Δ⁹-THC and/or DEX. HEK293T cells (5×10⁶ cells) were preseeded in a 6 well plate in growth medium overnight. The cells were then transiently transfected with pGRE-luc or pTAL-luc (vector control plasmid). After transfection, cells were treated with 0.1 % EtOH (VH), 0.5 μM DEX, different concentration of Δ⁹-THC (1, 5, and 10 μM), or the combination of 0.5 μM DEX plus 10 μM Δ⁹-THC. 24 h after transfection, luciferase activity was quantified in CPS and normalized to total protein (μg). The results are the mean±SEM. The “ns” indicates no significant difference. Results are representative of two separate experiments with three replicates per treatment group