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. 2004 Feb 20;101(9):3242–3247. doi: 10.1073/pnas.0306552101

Fig. 3.

Fig. 3.

Effect of bis-POM-PMEA on cytokine production by BMMΦ. (A) Effect of ET on LPS-induced TNF-α production. (B) Bis-POM-PMEA reversed the suppressive effect of ET on LPS-induced TNF-α production. (C) Effect of ET on IL-6 production. (D) Bis-POM-PMEA blocked ET-induced IL-6 production. To study the effect of ET on cytokine production, BMMΦ were treated with indicated amounts of EF and a 10-fold higher concentration of PA in the presence (A) or absence (C) of LPS. To examine the effect of PMEA (B and D), BMMΦ were pretreated with various concentrations of bis-POM-PMEA as described for Fig. 2. Cells were then stimulated with ET (12 ng/ml EF and 120 ng/ml PA) and 5 μg/ml LPS (B) or 30 ng/ml EF and 300 ng/ml PA (D) and received a second drug treatment. Culture supernatants were harvested 20 h later, and the amount of TNF-α (A and B) or IL-6 (C and D) was measured by using ELISA. Mean ± SE is representative of at least two experiments.