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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: Reproduction. 2011 Feb 10;141(5):653–663. doi: 10.1530/REP-10-0493

Figure 5.

Figure 5

Expression of macrophage markers in the epididymis. F4/80 immunolabeling of CD11c-EYFP mouse epididymis revealed 3 subsets of cells that express CD11c (green), F4/80 (red), or both markers (yellow). CD11c-F4/80+ cells (a, c, e, arrowheads) tend to be more interstitial than CD11c+F4/80− cells. However, high magnification pictures d and e show that cells positive for both markers are also present in the peritubular region. (f-h): the macrophage marker CD206 (mannose receptor) is expressed exclusively by interstitial cells. Sections of CD11c-EYFP (green) mouse epididymis were immunolabeled with an anti-CD206 antibody (red). While CD11c+ cells are located mostly in the peritubular region, the CD206+ macrophages are exclusively interstitial. Higher magnification (h) reveals the non-stellate morphology of interstitial CD206+ cells, contrasting with eDCs (green). DNA staining (in a, blue) helps to appreciate the structure of the tissue, with rows of nuclei from epithelial cells (E) and luminal nuclei from spermatozoa. Bars = 50 (a-c, f), 25 (g) and 10 µm (d, e, h). L = lumen.