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. 2004 Feb 20;101(9):3258–3263. doi: 10.1073/pnas.0308744101

Fig. 4.

Fig. 4.

Decreasing serum free leptin level increases bone mass. (A) Leptin-induced STAT3 reporter activity is decreased by ObRe treatment. (B) Schematic representation of the ApoE-ObRe transgene. Northern blot analysis confirmed the expression of the transgene in liver. (C) Size fractionation chromatogram. Marked reduction in serum free leptin level in Ob/+; ApoE-ObRe mice compared to Ob/+ mice (a representative chromatogram is shown, n = 4). (D and E) Three-month-old Ob/+; ApoE-ObRe mice had a significant increase in gonadal fat pad weight (D) and in bone volume over tissue volume (BV/TV, %) (E) compared to controls (n = 8; *, P < 0.05).