Fig. 2.

DA2 samples with focal 8q gains identified by aCGH share a common centromeric breakpoint within MYBL1. (A) aCGH data for the five DA2 samples with 8q gain, magnified on the right. Red: copy-number gain; green: loss. The highlighted sample (PLGG27, blue box) exhibits chromothripsis of chr8 but shows no other copy-number changes on other chromosomes. (B) FISH probes corresponding to sequences immediately distal (red) and proximal (green) of MYBL1 confirm that the single-copy gain identified by aCGH is a duplication involving one MYBL1 allele. The D8Z2 centromere enumeration probe (aqua) was used as a control. (C) Schematic representation of the proto-oncogene MYB family and breakpoints observed in our cohort in relation to the viral oncogene v-MYB.