Fig. 4.

Characterization of the DTF1 protein. (A) DTF1 directly interacts with CLSY1. Yeast two-hybrid assay for interactions among DTF1, CLSY1, and RDR2. Positive interactions are indicated by above-background growth of cells on the synthetic dropout medium without His, Leu, Trp, and supplemented with 3-AT. (B) Anti-GST Western blot for the GST–pull-down assay. Interaction between the GST–SAWADEE fusion protein and histone peptides is indicated by a positive signal on the Western blot and “input” was used as a control.