Fig. 2.

SV replenishment in intersectin 1 KO mice. (A) Simultaneous recordings of the presynaptic and postsynaptic compartments at the calyx of Held synapse. A pair of depolarizing pulses (0 mV for 50 ms, after a prepulse to +70 mV for 2 ms) was applied to the presynaptic terminal with an interval of 500 ms. During a 0-mV period, presynaptic Ca current was elicited. Evoked postsynaptic EPSCs (dotted line, first stimulation; solid line, second stimulation with an interval of 500 ms) and the cumulative release are shown. Cumulative release in response to the first pulse showed a double-exponential time course. (Upper) WT. (Lower) Intersectin 1 KO littermates. (B and C) Recovery of the fast (FRP; B) and the slow (SRP; C) components of release plotted against the stimulation interval. Dotted and solid circles represent data from control (four WT cells and four +/− cells) and KO (eight cells) mice, respectively. (D) The capacitance trace in response to a 50-ms pulse was normalized to the peak value and averaged among cells. Open and filled circles represent data from control and KO mice, respectively (n = 6 cells each). (E) The pulse duration increased to 500 ms. Note that for the open circle data, only downward error bars are displayed. Upward error bars are shown for the closed circle data for clarity.