Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 30;110(20):8266–8271. doi: 10.1073/pnas.1219234110

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Fig. 4. — Acute perturbation of the intersectin 1 SH3A domain slows SV replenishment. A pair of depolarizing pulses (0 mV for 50 ms after a prepulse to +70 mV for 2 ms) was applied to the presynaptic terminal with an interval of 500 ms. (A–C) EPSCs (dotted line, first stimulation; solid line, second stimulation, with an interval of 500 ms) and cumulative release (cum rel) are shown. (A) Control conditions. (B and C) Terminals dialyzed with SH3A domain (5 μM) (B) or antibodies directed against intersectin 1 SH3A (2,030 μg/mL) (C). (D) Similar experiments as in A–C, but with the stimulus interval of the two pulses varied. Recovery of the FRP (Left) and the SRP (Right) after RRP depletion was plotted against the ISI. Data from control conditions (open circles) and obtained in the presence of SH3A (filled circles), mutant SH3A (filled squares), or antibodies against the intersectin 1 SH3A domain (filled triangles) are shown. (E) Recovery of the fast-releasing component is plotted against ISI. The proline-rich domain of dynamin (filled triangles), a dynamin 1-derived proline-rich peptide (filled circles), or the proline-rich domain of N-WASP (filled squares) were introduced into the terminal (ISI = 0.5 and 1 s). Open circles, control condition. (F) Same as in E, except that the SH3E domain of intersectin 1 was introduced into the terminal (filled circles). Open circles, control condition.