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. 2013 Apr 30;110(20):8266–8271. doi: 10.1073/pnas.1219234110

Fig. 5.

Fig. 5.

Blocking the activity of the intersectin binding partner CDC42 impairs recruitment of release-ready SVs. (A) Simultaneous recordings of the pre- and postsynaptic compartments at the calyx of Held synapse (as depicted in Figs. 2 and 4). The DH-PH domain of intersectin 1 was introduced into the terminal. (Left) panel is similar to Fig 4 and the traces from one cell pair are shown (dotted: first stimulation, solid: second stimulation with an interval of 500 ms). Right) Plot of recovery of the fast component (FRP) against the stimulus interval. Filled circles, Dbl homology-pleckstrin homology (DH-PH) domain; open circles, control condition. (B) Same as in A, except with 1 μM toxin B introduced into the presynaptic terminal. Toxin B potently inhibits Rho family small G proteins. Open and filled circles represent the data from controls and in the presence of toxin B, respectively. (C) Same as in A, except that 20 μM secramine A (filled circles), a specific small-molecule inhibitor of CDC42, was applied to the terminal.