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Defect in the capacity of c-Jun(A266S) to activate expression of EBV early protein EA-D can be complemented by ZEBRA mutant Z(S186A). BZKO cells were transfected with plasmids encoding wt or mutant c-Jun(A266S) in the presence or absence of Z(S186A), a ZEBRA mutant that by itself is unable to activate the lytic cycle. The transfected cells were examined by Western blot for Rta, EA-D, and BGLF5 early proteins and BFRF3 late protein.
