Fig. 4.

Glycolytic activity regulates heme biosynthesis. S. aureus was grown in triplicate in the presence of vehicle or 10 µM ‘882. Heme levels were quantified using the pyridine hemochromogen assay and normalized to the concentration of protein in the whole cell lysates. (A) Cultures were treated with the indicated dose of 2-deoxyglucose (2dG). *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001. (B) Wild-type (WT) and ΔpfkA Newman were cultured in TSB + 1% pyruvate. #, the signal was below the limit of detection. (A and B) Error bars represent the SEM from three independent experiments. Statistical significance was determined using an unpaired Student’s t test.