Table 2.
Mutation rates of msh3 alleles.
| Tetranucleotide repeata | Dinucleotide repeatb | ||||
|---|---|---|---|---|---|
| Strain | Allele on plasmid |
Mutation ratec (x10−6) |
Fold change |
Mutation rate (x10−5) |
Fold change |
| FY23 (MSH3) | − | 10.1 (8.3–12.9) |
1 | NDd | - |
| EAY420 (msh3Δ) | − | 559.3 (440–1192.7) |
55.4 | ND | - |
| EAY420 | MSH3 | 6.0 (5.3–6.49) |
1 | 1.4 (1.1–1.9) |
1 |
| EAY420 |
Empty Vector |
346.4 (304–423.5) |
57.7 | 36.16 (27.5–42.6) |
26.3 |
| Transmitter region: | |||||
| EAY420 | msh3R744A | 175.3 (127.7–476.2) |
29.2 | 23.02 (18–26.7) |
16.7 |
| EAY420 | msh3R744L | 369.2 (293.5–409.0) |
61.9 | ND | - |
| EAY420 | msh3P745A | 22.7 (19–36.6) |
3.8 | ND | - |
| EAY420 | msh3P745L | 332.3 (302.7–436.9) |
55.3 | ND | - |
| EAY420 | msh3R761A | 20.45 (18–24.2) |
3.4 | ND | - |
| EAY420 | msh3N762A | 24.2 (18.7–54.9) |
4.0 | ND | - |
| EAY420 | msh3N762L | 26.2 (22.3–33.2) |
4.4 | ND | - |
| Walker A residues: | |||||
| EAY420 | msh3G795A | 208.5 (184.8–236.6) |
34.8 | 42.6 (32.9–47.0 |
30.9 |
| EAY420 | msh3G795D | 17.1 (14.2–23.0) |
2.9 | ND | - |
| EAY420 | msh3G796A | 678.4 (569.6–1087.9) |
113.0 | 228.8 (202.9– 283.5) |
166.2 |
| EAY420 | msh3G796D | 415.8 (289.5–555.2) |
69.3 | ND | - |
| Aromatic residues: | |||||
| EAY420 | msh3P774A | 13.6 (11.2–24.3) |
2.3 | ND | - |
| EAY420 | msh3P774L | 7.4 (6.0–10.1) |
1.2 | ND | - |
| EAY420 | msh3Y925A | 469.5 (444.8–503.3) |
78.3 | ND | - |
| EAY420 | msh3F940A | 343.2 (278.7–644.1) |
57.2 | 29.8 (16.7–59.9) |
21.7 |
| EAY420 | msh3Y942A | 180.1 (148.2–318.7) |
30.0 | 35.2 (32.5–43.5) |
25.1 |
a
The rate of slippage events within a tetranucleotide repeat were determined by transforming each strain with pBK14, which encodes a (CAGT)16 repeat in frame with the URA3 reporter gene. Slippage events are selected in the presence of 5-FOA.
b
The rate of slippage events within a dinucleotide repeat were determined by transforming each strain with pSH444, which encodes a (GT)16.5 repeat in frame with the URA3 reporter gene. Slippage events are selected in the presence of 5-FOA.
c
Mutation rates were determined as described in the Materials and Methods. Numbers in parentheses represent 95% confidence intervals.
d
ND = Not Determined