Skip to main content
. 2012 Sep 4;3:1034. doi: 10.1038/ncomms2023

Figure 4. Inhibition of in vitro ATP-mediated MC activation by 1F11 mAb.

Figure 4

(a) BM-derived MCs, pretreated with various concentrations of 1F11 mAb (0, 1, 10 μg ml−1) for 15 min, were stimulated with 0.5 mM ATP for 30 min. Cells were stained with an anti-CD63 mAb for flow cytometric analysis. Data are representative of three independent experiments. (b) BM-derived MCs pretreated with various concentrations of 1F11 mAb or control rat IgG2b (0, 10 μg ml−1) for 15 min were stimulated with 0.5 mM ATP for 30 min in the presence of 1 mg ml-1 Lucifer yellow (LY). (c) LY uptake was determined by using flow cytometry and fluorescence microscopy. Scale bar, 100 μm. Data are representative of three individual experiments.