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Z-contrast STEM image of uncontrasted MIN6 Islet beta cells embedded in LR White resin, (a) The thickness of the ultrathin section is ca. 60 nm, (b) of the thick section ca. 200 nm. The nucleus (Nu) with dense heterochromatin and less dense euchromatin, and mitochondria (arrows) are visible in both images. The electron dose is about 86 e−/Å2 per frame (200 kV, 75 um condenser aperture, probe current of 691 pA, pixel dwell time of 50 μs and pixel size of 25 nm2).
