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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Ultramicroscopy. 2013 Feb 4;128:24–31. doi: 10.1016/j.ultramic.2013.01.004

Figure 13.

Figure 13

(a) Cryo-STEM image of TMV virus collected by bright-field detector at wide-angle mode with a large collecting angle (up to 30 mrad). (b) An integrated line profile of the rectangular area shown in (a), from position O to E. Besides the two back lines corresponding to the edges of the TMV virus, a thin black line can also be found corresponding to the low-density center, as marked by the arrowheads. (c) is the structure of a TMV virus projected along the helical axis showing there is a low-density area in the center. The electron dose for imaging is about 53 e−/Å2 per scan (80 kV, 50 um condenser aperture, probe current of 84 pA, pixel dwell time of 10 μs and pixel size of 98 Å2).