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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Anal Biochem. 2012 Sep 19;432(1):49–57. doi: 10.1016/j.ab.2012.08.031

Fig. 1. SDS-PAGE analysis.

Fig. 1

Profiles obtained for (1) affinity-purified Bd-cys, (2) Bd-cys-S-FL, (3) FL-labeled Bd-cys dimer, and (4) Bd-cys-S-PEG3400-biotin. Samples were prepared in buffer lacking β-ME (A) or containing 5% (v/v) β-ME (B) immediately prior to electrophoresis (4–20% acrylamide Tris-HCl gel). MW: molecular weight (kDa) of standards. Dimer bands were present in the profiles of all three conjugates (lanes 2–4) in non-reducing conditions (A), but not when the loading buffer contained β-ME (B).