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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Anal Biochem. 2012 Sep 19;432(1):49–57. doi: 10.1016/j.ab.2012.08.031

Fig. 4. SPR sensograms (RU) for kinetics of binding of anti-GABAA-ρ1 and anti-GABAA-α1 to immobilized Bd-cys-S-PEG3400-biotin.

Fig. 4

A: Red: sensograms obtained with anti-GABAA-ρ1 at 20, 10, 5, 2.5, 1.25, and 0.625 nM. Black: fitted curves obtained using Biacore software in a 1:1 binding analysis. B: Sensograms (red) and fitted curves (black) obtained with the addition of anti-GABAA- α1 at 10, 5, 2.5, 1.25 and 0.625. nM. Binding kinetics of both antibodies to Bd-cys-S-PEG3400-biotin are well described by Langmuir 1:1 binding analysis (see Methods).