Table 1. Kinetic parameters.

Rows 1–3: SPR determinations of binding constant (K_D), association rate constant (k_a), and dissociation rate constant (k_d), for the binding of anti-GABA_A-ρ1 and anti-GABA_A-α1 to Bd-cys-S-PEG3400-biotin capture agent. Data for a given antibody were obtained in 3 experiments, each involving triplicate samples at each investigated antibody concentration. Rows 4–5: FA determinations of EC50 for binding of Bd-cys-S-FL monomer and FL-labeled Bd-cys dimer to anti-GABA_A-ρ1 and anti-GABA_A-α1. EC₅₀ values derived from eq. 1 fitting to data obtained in 1 (anti-GABA_A-ρ1) or 2 (anti-GABA_A-α1) experiments, each involving triplicate samples at each of 14 concentrations of antibody.

Row	Method	Preparation	Parameter	Anti-GABAA-ρ1	Anti-GABAA-α1
1	SPR	Bd-cys-S-PEG3400-biotin	KD (nM)	6.4 ± 1.9	0.4 ± 0.1
2	SPR	Bd-cys-S-PEG3400-biotin	ka (M−1 s−1)	2.1 × 105 ± 0.3 × 105	1.3 × 106 ± 5.0 × 104
3	SPR	Bd-cys-S-PEG3400-biotin	kd (s−1)	1.3 × 10−3 ± 2.3 × 10−4	4.8 × 10−4 ± 1.0 × 10−4
4	FA	Bd-cys-S-FL	EC50 (nM)	65	18
5	FA	FL-labeled Bd-cys dimer	EC50 (nM)	93	25