Figure 1.
Adhesion of cells expressing different carbon-binding peptides on their flagella to carbon-coated surfaces. (A) E. coli GI826 cells harboring plasmids encoding the indicated FliC∷Trx∷Car fusions (where Car denotes a carbon binding peptide) were contacted with carbon-coated cover slips. After washing, slides were transferred to the stage of a microscope and the number of cells present in a field was counted at 500-fold magnification. Error bars were obtained for 5 different fields and two separate experiments. (B) Characteristic appearance of carbon-coated slides following incubation with cells expressing low (Car14, Car16), moderate (Car15) or high avidity (Car9) carbon binding peptides on their flagella.