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SPR analysis of the adsorption of carbon-binding TrxA derivatives to amorphous carbon.. (A) Authentic TrxA (grey), TrxA∷Car9 (green) or TrxA∷Car15 variants (blue) were flowed over a carbon-coated SPR chip at 1 µM concentration. Pure buffer was flowed after 15 min (arrows). Sensograms were obtained at the indicated concentrations of TrxA∷Car9 (B) or TrxA∷Car15 (C) for Kd calculation. See text and supplementary information for details.
