Fig. 4.

FGF21 levels are altered in HFD-fed mice treated with exendin 4 (Ex4). a Plasma FGF-21 levels and (b) relative Fgf21 RNA expression in the liver measured by quantitative RT-PCR and normalised to housekeeping TATA-box binding protein. c FGF21 protein levels in the liver normalised to actin. d Correlation of liver FGF21 protein levels with plasma FGF21 (white circles, HFD saline; black circles HFD exendin 4). e Correlation of liver FGF21 protein levels with liver weight as per cent of body weight. f Blot showing abundance of phosphorylated (P) AMPK and ACC in the liver of HFD-saline and HFD-exendin 4 mice, with quantification of AMPK and ACC. n=4 per group; *p<0.05 one-tailed t test; ^†p<0.05, **p< 0.01 two-tailed t test