Table 1.

Abbreviations, conditions and summary of results for key experiments.^A

Target peptide on cancer cells				Hosts		T cells		Tumor rejection
Desig- nation	Sequence	MHC	Affinity of peptide for MHC (IC50 [nM])B	Desig- nation	Relationship of antigen to recipient	Desig- nation	Relationship of antigen to donor
SIY	SIYRYYGL	Kb	1.1	OT-I	non-self	2C	non-self	5/5C,D,E,F
						none		0/6C
OVA257–264	SIINFEKL		0.9	2C	non-self	OT-I	non-self	4/4G
						none		0/4G
Tyr369–377	FMDGTMSQV	A2	4.2H	OTA	self	FH	self	6/7I
						none		0/5I
hgp10025–33	KVPRNQDWLJ	Db	186	OT-I	non-self	pmel	non-self	1/8D
						none		0/2
EGP	EGPRNQDWL		454	OT-I	non-self	pmel	non-self	1/6E
						none		0/5
mgp10025–33	EGSRNQDWL		22,975	OT-I	self	pmel	self	1/12F
						none		0/6

^ASee Table S1 for details.

^BIC₅₀ values represent the geometric mean of 5 or more experiments.

^Cp = 0.002;

^Dp < 0.005;

^Ep = 0.015;

^Fp < 0.001;

^Gp < 0.029;

^HA higher IC₅₀ value of 65 nM was published for this peptide earlier (Colella et al., 2000). The differences in affinity measurements likely arose as a result of small differences in reagents, methodology, and procedures.

^Ip = 0.015

^Jonly the underlined amino acids differ between the three gp100 peptide variants