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Experimental validation of predicted endocytosis functions in human. (A–C) Co-localization of SH3D19-GFP (C-terminal tag) with CLTA-TagRFP-TEN-1 in SK-MEL-2 cells. (D–F) Co-localization of GFP-MAP3K9 (N-terminal tag) with CLTA-TagRFP-TEN-1. (G, H) Localization of GFP-CTTNBP2NL and GFP-BZRAP1 to actin stress fibre-like structures. (I) Localization of GFP-FGR to focal adhesion-like structures. Scale bars, 10 μm.
