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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Immunity. 2012 Apr 12;36(5):742–754. doi: 10.1016/j.immuni.2012.03.012

Figure 7. NLRP12 attenuates noncanonical NF-κB regulated chemokines, cancer associated gene expression and specific MAPK family members during CAC.

Figure 7

(A) Cxcl12 and Cxcl13 gene expression in colons. (B) Colon levels of CXCL13 were assessed from organ culture supernatants by ELISA. (A-B) WT mock, n = 5; Mock/DSS WT, n = 3; AOM/Mock WT, n = 3; AOM/DSS Nlrp12-/-, n = 5; AOM/DSS WT, n = 15. (C) Macroscopic polyps were removed from wild type and Nlrp12-/- mice and gene expression was assessed using a multiplex gene expression array. Data shown represent genes that were significantly up-regulated, defined as >2 fold increase, in RNA that was pooled from polyps that were microdissected from Nlrp12-/- mice compared to wild type mice. WT, n = 5; Nlrp12-/-, n = 5. (D) Gene expression for Akt1, Jun and Nr3c1 was used to verify the array data using non-pooled RNA collected from the whole colons of additional mice that were not assessed on the array. WT mock, n = 3; Nlrp12-/- mock, n = 3; WT DSS, n = 3; WT AOM, n = 3; AOM/DSS Nlrp12-/-, n = 5; AOM/DSS WT, n = 5. (E) pERK levels were evaluated by immunohistochemistry from paraffin embedded colon sections. (F) pERK levels were evaluated using semiquantitative histopathology image analysis (ImageJ). AOM/Mock WT, n = 3; AOM/DSS Nlrp12-/-, n = 6; AOM/DSS WT, n = 7.