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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: J Immunol Methods. 2008 Nov 17;340(2):144–153. doi: 10.1016/j.jim.2008.10.013

Figure 2. Identification of anti-hTLR5 or anti-hTLR6 antibody clones.

Figure 2

(A&B) Screening anti-hTLR5 (A) or anti-hTLR6 (B) antibody clones. Individual clones of raw phage preparation were used as primary Ab to stain hTLR5- (A, white), hTLR6- (B, white), or YFP- (A & B, gray) HEK cells. 20 representative clones were shown here. Positive clones are indicated in bold. (C&D) Flow cytometry staining results for two representative hTLR5 (C) or hTLR6 (D) antibody clones. Raw phage preparations (left), purified phage (center), or purified scFvs (right) were used as primary antibody to stain hTLR5- (C, white), hTLR6- (D, white) or YFP- (gray) HEK cells.