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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: J Immunol Methods. 2008 Nov 17;340(2):144–153. doi: 10.1016/j.jim.2008.10.013

Figure 3. Anti-TLR antibody clones specifically bind their target Ag but not other TLRs.

Figure 3

scFvs against hTLR2 (clone B11), hTLR5 (clone 3B5), hTLR6 (clone 3F7), mTLR2 (clone B2–3), or Tie2 extra-cellular domain (ExTek, clone 1D6) were used as primary antibodies to stain hTLR1-, hTLR2-, hTLR4-, hTLR5-, hTLR6-, mTLR2- or mTLR5-HEK (white), with YFP-HEK as negative control cells (gray). All scFv clones obtained to date show the same specificity to their target TLR Ag and the result of one representative clone is shown here for each group. Positive stainings are outlined in bold.