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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: J Immunol Methods. 2008 Nov 17;340(2):144–153. doi: 10.1016/j.jim.2008.10.013

Figure 4. Sera from hTLR2-immunized mice does not contain detectable levels of IgG against hTLR1, hTLR5, hTLR6 or mTLR2.

Figure 4

Mice were immunized with hTLR2 by a cell-based immunization method and sera were harvested 7 days after the 5th boost. Mice sera were used as primary staining reagents in flow cytometry to test their binding on hTLR2-, mTLR2-, hTLR5-, hTLR6- or hTLR1-HEK, respectively. Ag-specific IgG titer was detected by secondary staining of APC-conjugated goat-anti-mouse IgG antibody. Serum from naïve mice were used as negative control.