Table 1.
Summary of numbers of clones and positive rates for screening/verification at each step of anti-TLR scFv generation.
| Screened by | Initial Screen - Raw Phage | Fingerprint | Purified phage Binding to cells | scFv binding to cells | |
|---|---|---|---|---|---|
|
| |||||
| # of clones screened | # of clones positive | # of unique clones | # of clones positive | # of clones positive | |
| hTLR2 HEK | 168 | 78 (46%) | 9/23 (39%) | 8/9 (89%) | 5/5 (100%) |
|
| |||||
| mTLR2-HEK | 84 | 43 (51%) | 18/32 (56%) | 5/9 (56%) | 5/5 (100%) |
|
| |||||
| hTLR5-HEK R3 | 186 | 39 (21%) | 18/39 (46%) | 2/17 (12%) | 1/2 (50%) |
|
|
|||||
| R4* | 92 | 22 (24%) | 2/19 (11%) | 1/2 (50%) | 1/2 (50%) |
|
| |||||
| hTLR6-HEK | 186 | 11 (6%) | 6/8 (75%) | 1/6 (17%) | 1/1 (100%) |
|
| |||||
| hTLR1-HEK | 94 | 0 (0%) | N/A | N/A | N/A |
Numbers in fractions indicate “number of positive clones/number of clones tested” and percentages in parentheses indicate positive rates for each test.
*
Results after performing a 4th round of selection (R4) for hTLR5 versus the standard 3 rounds (R3) of selection.