Table 2.

Differentially Expressed Genes over Four Hours in Three Temperature Conditions

Univariate Analysis
Temperature	Time	Number of Differentially Expressed Genes at each time point	P-value	FDR
4C	5 mins 30 mins 60 mins 120 mins 240 mins	30 6 29 32 633	<0.001 <0.001 <0.001 <0.001 <0.001	0.99 0.96 0.94 0.94 <0.08
22C	5 mins 30 mins 60 mins 120 mins	15 164 469 352	<0.001 <0.001 <0.001 <0.001	0.91 <0.29 <0.11 <0.15
37C	5 mins 30 mins 60 mins 120 mins	387 375 1048 3945	<0.001 <0.001 <0.001 <0.001	<0.14 <0.14 <0.05 <0.01

Multivariate Analysis using a Blocking Variable
Temperature	Time	Number of Differentially Expressed Genes across all time points	P-value	FDR
4C	All time points	130	<0.001	<0.40
22C	All time points	302	<0.001	<0.18
37C	All time points	4797	<0.001	<0.01