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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: Gastroenterology. 2011 Sep 16;141(6):2064–2075. doi: 10.1053/j.gastro.2011.08.048

Figure 2.

Figure 2

Long-term (24 hr) A/A-induced NMDA channel activation reduced cell viability and regulated the transcription of apoptosis genes. (A) Quantification of fluo-3 signal in vacuoles at 120 min. At the end of microscopy experiments, cell viability was measured. (B) Cell viability at 24 hr with A/A and MK-801 or ifenprodil (Ifen). MKN28 cells were transfected with the NMDAR2B expression plasmid (NR2B+) and incubated without HP (None) or with 5 mM urea and wild-type (WT) HP or its isogenic ureB mutant (ureB−). (C) Cells were incubated with A/A and BAPTA-AM (B-AM), MK-801 (MK), or ifenprodil (Ifen). Band intensity for BAX or BAK was quantified as a ratio to beta-actin. n ≥ 3 experiments per condition; *P < 0.05, ** P < 0.01, *** P < 0.001, ND, not different; comparisons as indicated by brackets.