Table 3.
Differentiation phenotypes of peptide-specific CD8+tet+ and CD8+tet− T cells determined at baseline and on Days 43 and 85 after vaccination1
| CD8+tet+ | N | EM | CM | TD |
|---|---|---|---|---|
| gp100 | ||||
| Baseline | 50 (13–86) | 7 (0–38) | 29 (4–61) | 8 (0–41) |
| Day 43 | 40 (0.6–75) | 12 (0–90) | 28 (1.6–75) | 8 (0–59) |
| Day 85 | 34 (0.8–56.5) | 22 (0–80) | 27 (0.5–54) | 8 (0–95) |
| p < 0.076 | p < 0.048 | NSD | NSD | |
| MART-1 | ||||
| Baseline | 44 (11–71) | 11 (1–59) | 31 (4–57) | 8 (0–45) |
| Day 43 | 41 (0–79) | 17 (0–63) | 24 (8–70) | 11 (0–57) |
| Day 85 | 30 (5–70) | 18 (0–82) | 27 (5–71) | 13 (0–45) |
| p < 0.006 | p < 0.048 | NSD | NSD | |
| Tyrosinase | ||||
| Baseline | 47 (4–88) | 6 (0–39) | 29 (4–73) | 7 (4–73) |
| Day 43 | 42 (7–93) | 7 (0–30) | 32 (0–82) | 8 (0–76) |
| Day 85 | 51 (7–87) | 6 (0–47) | 30 (4–75) | 5 (0–75) |
| p < 0.014 | ||||
| FLU | ||||
| Baseline | 34 (9–73) | 18 (2–55) | 28 (4–57) | 15 (0–53) |
| Day 43 | 30 (0–61) | 17 (4–73) | 28 (0–70) | 15 (0–52) |
| Day 85 | 30 (8–57) | 17 (3–65) | 29 (6–78) | 15 (0–57) |
| CD8+tet− | ||||
| Baseline | 18 (5–63) | 30 (14–61) | 9 (2–27) | 38 (13–65) |
| Day 43 | 23 (4–59) | 29 (14–51) | 11 (2–30) | 35 (18–63) |
| Day 85 | 18 (5–61) | 32 (9–51) | 13 (2–46) | 40 (9–61) |
1
The data are median percentages of positive cells among all CD8+tet+ T cells or all CD8+tetneg T cells with the ranges in parentheses. At baseline, the differentiation phenotypes were determined in 37, on Day 43 in 35, and on Day 85 in 21 patients. Naïve T cells (N) are defined as CD45RA+CCR7+; effector memory T cells (E) as CD45RA−CCR7−, central memory (CM) as CD45RA−CCR7+ and terminally differentiating (TD) as CD45RA+CCR7−.