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. 1971 Feb;47(2):217–221. doi: 10.1104/pp.47.2.217

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation 1

Aurea Vasconcelos a, Marilyn Pollack a, Leticia R Mendiola a, H-P Hoffmann a,2, D H Brown a,3, C A Price a
PMCID: PMC365845  PMID: 16657599

Abstract

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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