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. Author manuscript; available in PMC: 2013 May 20.
Published in final edited form as: Methods Mol Biol. 2012;798:395–410. doi: 10.1007/978-1-61779-343-1_23

Fig. 2.

Fig. 2

(a) A xy field scan image of a permeabilized EDL muscle fiber loaded with the internal solution containing 100 μM fluo-4, 200 nM free Ca2+, and 2 mM free Mg2+. (b) A xt line scan image of the same muscle fiber obtained through repeatedly scanning the fiber along the dashed line. Traces a, b, and c are the quantitative measurement of Ca2+ sparks by evalu-2+ sparks.