Table 3.
The quantitative proteomic evaluation of LC-MS/MS analyses of 30 bound fractions collected from lectin enrichment experiments using two different affinity media. All areas were calculated using ProteinQuant, a quantitative proteomic computer tool [45].
| Index | Protein | Description | Mass | Lectin matrix A | Lectin matrix B | ||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Normalized area, confidence interval (95%) (×10-4) | RSD [%] |
Normalized area, confidence interval (95%) (×10-4) | RSD [%] |
||||
| 1 | A1AG1_HUMAN | (P02763) Alpha-1-acid glycoprotein 1 | 23725 | 56 ± 4 | 37 | 68 ± 6 | 23 |
| 2 | A1BG_HUMAN | (P04217) Alpha-1B-glycoprotein | 54809 | 192 ± 5 | 7 | 160 ± 9 | 16 |
| 3 | A2MG_HUMAN | (P01023) Alpha-2-macroglobulin | 164600 | 2449 ± 25 | 3 | 2761 ± 45 | 5 |
| 4 | AACT_HUMAN | (P01011) Alpha-1-antichymotrypsin | 47792 | 69 ± 4 | 17 | 20 ± 2 | 22 |
| 5 | AFAM_HUMAN | (P43652) Afamin | 70963 | 26 ± 2 | 17 | - | - |
| 6 | AMBP_HUMAN | (P02760) AMBP protein | 39886 | - | - | 33 ± 3 | 26 |
| 7 | ANGT_HUMAN | (P01019) Angiotensinogen | 53406 | 53 ± 2 | 12 | 36 ± 3 | 20 |
| 8 | ANT3_HUMAN | (P01008) Antithrombin-III | 53025 | 61 ± 3 | 12 | 33 ± 3 | 21 |
| 9 | APOA1_HUMAN | (P02647) Apolipoprotein A-I | 30759 | 110 ± 6 | 14 | 108 ± 11 | 30 |
| 10 | C1R_HUMAN | (P00736) Complement C1r subcomponent | 81661 | - | - | 43 ± 2 | 16 |
| 11 | C4BP_HUMAN | (P04003) C4b-binding protein alpha chain | 69042 | 123 ± 4 | 10 | 152 ± 8 | 14 |
| 12 | CERU_HUMAN | (P00450) Ceruloplasmin (EC 1.16.3.1) | 122983 | 441 ± 8 | 7 | 433 ± 12 | 8 |
| 13 | CFAB_HUMAN | (P00751) Complement factor B | 86847 | 159 ± 4 | 7 | 124 ± 7 | 16 |
| 14 | CFAH_HUMAN | (P08603) Complement factor H | 143654 | 244 ± 5 | 5 | 293 ± 14 | 13 |
| 15 | CLUS_HUMAN | (P10909) Clusterin | 53031 | 20 ± 1 | 12 | - | - |
| 16 | CO3_HUMAN | (P01024) Complement C3 | 188585 | 1878 ± 33 | 5 | 1948 ± 54 | 8 |
| 17 | CO5_HUMAN | (P01031) Complement C5 | 189923 | 18 ± 1 | 13 | - | - |
| 18 | CO6_HUMAN | (P13671) Complement component C6 | 108425 | 16 ± 1 | 17 | 25 ± 16 | 183 |
| 19 | CPN2_HUMAN | (P22792) Carboxypeptidase N subunit 2 | 61431 | 25 ± 1 | 16 | 28 ± 3 | 32 |
| 20 | HEMO_HUMAN | (P02790) Hemopexin | 52385 | 728 ± 18 | 7 | 611 ± 37 | 17 |
| 21 | HEP2_HUMAN | (P05546) Heparin cofactor 2 | 57205 | 31 ± 3 | 26 | - | - |
| 22 | HPT_HUMAN | (P00738) Haptoglobin | 45861 | 221 ± 9 | 12 | 285 ± 21 | 20 |
| 23 | HRG_HUMAN | (P04196) Histidine-rich glycoprotein | 60510 | - | - | 31 ± 2 | 22 |
| 24 | IC1_HUMAN | (P05155) Plasma protease C1 inhibitor | 55347 | 139 ± 9 | 18 | 130 ± 12 | 27 |
| 25 | IGHA1_HUMAN | (P01876) Ig alpha-1 chain C region | 38486 | 35 ± 2 | 15 | 57 ± 2 | 11 |
| 26 | IGJ_HUMAN | (P01591) Immunoglobulin J chain | 16041 | 2 ± 0.3 | 52 | 2 ± 0.4 | 55 |
| 27 | ITIH1_HUMAN | (P19827) Inter-alpha-trypsin inhibitor heavy chain H1 | 101782 | 143 ± 4 | 8 | 183 ± 7 | 11 |
| 28 | ITIH2_HUMAN | (P19823) Inter-alpha-trypsin inhibitor heavy chain H2 | 106826 | 95 ± 4 | 11 | 140 ± 7 | 14 |
| 29 | ITIH4_HUMAN | (Q14624) Inter-alpha-trypsin inhibitor heavy chain H4 | 103522 | 113 ± 3 | 8 | 106 ± 8 | 21 |
| 30 | KAC_HUMAN | (P01834) Ig kappa chain C region | 11773 | 55 ± 3 | 13 | 61 ± 9 | 39 |
| 31 | KNG1_HUMAN | (P01042) Kininogen-1 | 72984 | - | - | 47 ± 3 | 18 |
| 32 | LAC_HUMAN | (P01842) Ig lambda chain C regions | 11401 | 20 ± 1 | 17 | - | - |
| 33 | MUC_HUMAN | (P01871) Ig mu chain C region | 50210 | 84 ± 3 | 12 | 136 ± 6 | 11 |
| 34 | SAMP_HUMAN | (P02743) Serum amyloid P-component | 25485 | 63 ± 3 | 12 | 76 ± 8 | 31 |
| 35 | VTNC_HUMAN | (P04004) Vitronectin | 55069 | 85 ± 3 | 10 | 41 ± 2 | 16 |