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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Bioconjug Chem. 2013 Mar 27;24(4):520–532. doi: 10.1021/bc300665t

Table 2.

Triple-labeling of BSA and HSA through three different bioconjugation chemistries. Compounds numbers are shown in parenthesis. Estimated numbers of surface accessible amino acids (AAs) from ThermoScientific or estimated by PyMOL ver 1.5.

Protein Estimated number of available AAs/Total Number of residues labeled by Amidation Number of residues labeled by Tyrosine-click Number of residues labeled by Michael reaction
HSA Lys 30/55 4.1 (12) 6.5 (14a) 1.8 (16a)
Tyr 15/19 6.7 (14b) 1.2 (16b)
Cys 1/35 4.6 (14c) 1.6 (16c)
BSA Lys 35/60 3.8 (13) 8.3 (15a) 1.3 (17a)
Tyr 15/21 7.4 (15b) 2.3 (17b)
Cys 20/35 3.9 (15c) 2.7 (17c)