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. 2013 Apr 16;108(9):1817–1821. doi: 10.1038/bjc.2013.175

Figure 2.

Figure 2

Effect of miR-125b overexpression on OSCC-derived cell lines. A cellular proliferation assay was performed to investigate the effects of the overexpression of miR-125b on the OSCC-derived cell lines HSC-2 (A) and HSC-3 (B). *Significant differences between transfectants and negative controls (NCs; P<0.05, Student's t-test). The data are expressed as means±s.d. Cell survival after irradiation was examined with a clonogenic survival assay (C and D). After exposure to 2, 4, 6 and 8 Gy of X-ray irradiation, cells were seeded and cultured. The significance of the differences between transfectant and NC cells is indicated with asterisks (*) (P<0.05, Student's t-test). The data are expressed as means±s.d. The ICAM2 mRNA expression in miR-125b-transfected cells and NC cells in HSC-2 (E) and HSC-3 (F) is shown. The expression levels of ICAM2 mRNA were decreased after the introduction of miR-125b to both cell lines (P<0.01, Student's t-test). The data are expressed as means±s.d. The luciferase activity of the reporter gene containing the wild-type 3′-UTR of the ICAM2 gene (ICAM2-WT) or the mutated 3′-UTR of the ICAM2 gene (ICAM2-Mut) was determined (G). The data are expressed as means±s.d. Relative luciferase activity was significantly suppressed in the cells with ICAM2-WT when miR-125b was co-transfected (P=0.001, Student's t-test).