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. 2013 Apr 11;108(9):1862–1869. doi: 10.1038/bjc.2013.163

Figure 3.

Figure 3

Expressions of LIFR, ANXA11, and their regulatory proteins by the western blot in clones with LIFR rs3729740 wild-type allele (G allele, G1 and G2 clones), mutant allele (A allele, A1 and A2 alleles), and control RKO cells (−); ANXA11 expression in clones with ANXA11 rs1049550 minor allele (T allele, T1 and T2 clones), ancestral allele (C allele, C1 and C2 alleles), and control RKO cells (−), respectively (A and B). Empty vectors were used for control RKO cells, that is, HA-tagged pcDNA3 for LIFR and Myc/His-tagged pcDNA3 for ANXA11, respectively. RKO clones stably expressing wild-type (or ancestral) and mutant (or minor) alleles (upper rows) were incubated for 24 h with FXC for LIFR and FRB for ANXA11, respectively. β-Actin was used as a loading control. Values in each parenthesis indicate the molecular weight (kD) of relevant protein. Relative expression ratios of p-ERK and MMP-9 to β-actin were calculated between clones expressing respective alleles and control RKO cells (C and D, respectively). Values are means±s.e.m. of quadruplicates. P-values using Student's t-test are displayed over the bars. Bold font, P<0.05.