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. 2013 Apr 20;24(5):766–771. doi: 10.1021/bc3006418

Figure 2.

Figure 2

RU486-BODIPY nuclear accumulation is PR dependent. (a) RU486-BODIPY accumulates in the nuclei of PR positive cells but not PR negative cells. T47D (PR positive) or MDA-MB-231 (PR negative) cells were incubated with 5 nM RU486-BODIPY for 15 min, washed, and imaged after 45 min. (b) Nuclear accumulation of RU486-TAMRA in T47D cells can be competed off with PR agonist but not with GR agonist. T47D cells were coincubated with 5 nM RU486-BODIPY and 100 nM progesterone (PR agonist) or dexamethasone (GR agonist) for 15 min, washed, and imaged after 45 min. Scale bar 20 μm.