Table 1.
Identification of Colletotrichum using primers taxon-specific CaInt-ITS4, CgInt-ITS4 and Col-ITS4.
| Species | Strain number | PCR identification | ITS | ||
|---|---|---|---|---|---|
| CaInt-ITS4 | CgInt-ITS4 | Col-ITS4 | |||
| C. boninense | LGMF625 | − | − | − | C. boninense |
| LGMF666 | − | − | + | na | |
| C. acutatum | LGMF738 | + | − | − | C. acutatum |
| C. gloeosporioides | LGMF621 | − | + | − | na |
| LGMF622 | − | + | − | C. gloeosporioides | |
| LGMF623 | − | + | − | C. gloeosporioides | |
| LGMF626 | − | + | − | C. gloeosporioides | |
| LGMF627 | − | + | − | C. gloeosporioides | |
| LGMF633* | − | + | − | na | |
| LGMF638 | − | + | − | na | |
| LGMF667 | − | - | − | C. gloeosporioides | |
| LGMF669 | − | + | − | na | |
| LGMF672 | − | + | − | C. gloeosporioides | |
| LGMF678 | − | + | − | na | |
| LGMF680 | − | + | − | C. gloeosporioides | |
| LGMF682 | − | + | − | na | |
| LGMF686 | − | + | − | na | |
| LGMF687 | − | + | − | C. gloeosporioides | |
| LGMF688 | − | + | − | C. gloeosporioides | |
| LGMF689 | − | + | − | C. gloeosporioides | |
| LGMF721 | − | + | − | C. gloeosporioides | |
| LGMF722 | − | + | − | C. gloeosporioides | |
| LGMF723 | − | + | − | C. gloeosporioides | |
| LGMF726* | − | + | − | na | |
| LGMF727 | − | + | − | C. gloeosporioides | |
| LGMF728 | − | + | − | C. gloeosporioides | |
| LGMF729* | − | + | − | na | |
| LGMF730 | − | + | − | na | |
| LGMF731 | − | + | − | C. gloeosporioides | |
| LGMF732 | − | + | − | C. gloeosporioides | |
| LGMF733 | − | + | − | na | |
| LGMF734 | − | + | − | C. gloeosporioides | |
| LGMF735 | − | + | − | C. gloeosporioides | |
| LGMF736 | − | + | − | C. gloeosporioides | |
| LGMF740 | − | + | − | na | |
| LGMF741 | − | + | − | C. gloeosporioides | |
| LGMF742 | − | + | − | C. gloeosporioides | |
| LGMF743 | − | + | − | na | |
| LGMF744 | − | + | − | C. gloeosporioides | |
(*) dsRNA confirmed after analysis digestion with RNAse, DNAse, and S1 Nuclease. na: not available.