Table 1.
Multiplex PCR primer list.
| Name | Forward primer sequence | Reverse primer sequence | lane | size bp | R.E. | Digested fragment sizes |
|---|---|---|---|---|---|---|
| 6-1 | acgtggccttgtgcggtacagtc | agagacgagtcaggcaatgc | 4 | 757 | HaeIII | 259, 250, 101, 72, 68, 7 |
| 6-2 | tgtcccatctgcgcaccgaagac | cgtacactgtttgtgggcgcttc | 4 | 592 | AluI | 366, 129, 97 |
| 11-1 | agttccgtagatgccaagggca | tgcctcaggtgaggcccaatgc | 3 | 528 | RsaI | 194, 172, 94, 68 |
| 11-2 | tggtaccccctacacagggtgg | acagaatgttggacagggtcagg | 3 | 730 | HaeIII | 433, 160, 137 |
| 16-1 | ttaggcagcacttggccaacca | taatccgtcctttgtgtgagct | 3 | 207 | MspI | 110, 97 |
| 16-2 | actgcaatgtttcaggacccac | cgaagcgtagagtcacacttgc | 2 | 661 | MspI | 405, 199, 57 |
| 18-1 | tcgcgtcctttatcacagggcga | tgcccaggtacaggagactgtg | 1 | 536 | AluI | 235, 200, 101 |
| 18-2 | tccgtggtgtgcatcccagcag | cacttgtgcatcattgtggacc | 2 | 274 | RsaI | 185, 48, 41 |
| 26-1 | tggtatacaacgagtgtcagctcc | ggggcaatgatggccatgtcg | 7 | 635 | MspI | 425, 210 |
| 31-1 | aggcacggttggtgaatcggtc | tagatgctgagggtgcactacg | 1 | 683 | HaeIII | 481, 202 |
| 31-2 | catgaactaagctcggcattgg | tccaacatgctatgcaacgtcc | 2 | 385 | RsaI | 233, 152 |
| 33-1 | agcttagaggtgtggctttgtg | tgcagttagttgcagtacgtgc | 2 | 493 | RsaI | 211, 145, 122, 15 |
| 33-2 | tgacccacctacagctgcaatc | gggtgtgtacattatccacatcg | 2 | 899 | RsaI | 615, 277, 7 |
| 35-1 | ccaccaagtggttccaacgcag | tgtaggcgtgtagctgtgtagc | 3 | 488 | RsaI | 216, 193, 79 |
| 35-2 | gtcctgttggaaaccaacacgt | acacacagacgtagtgtcgcct | 3 | 251 | AluI | 135, 81, 35 |
| 39-1 | acacaaacggtgtattccgtgcca | tgtgcagttggagatttgggatcc | 5 | 200 | RsaI | 129, 71 |
| 39-2 | tgtgcagtaccagtgacggatcg | atttttggcgttgtgactctgtg | 6 | 438 | HaeIII | 221, 217 |
| 45-1 | ggacatcacacctaccgtggac | ctgtgaggtggacacacggacc | 4 | 298 | RsaI | 126, 69, 59, 26, 18 |
| 45-2 | acctgcacaattgcaacctggt | caactgccaggggtttcacgca | 4 | 345 | RsaI | 179, 105, 61 |
| 51-1 | aattgctggcaacgtacacgac | acacttgaacacctgcaacacg | 4 | 255 | RsaI | 190, 51, 14 |
| 51-2 | cctactccaggggttagtcgca | taaggagggcaactgcctagac | 4 | 504 | HaeIII | 270, 234 |
| 52-1 | cccaagtgtaacgtcatgcgtg | agggttgtttatagccgtgcac | 3 | 323 | AluI | 215, 108 |
| 52-2 | acctccgcagtgtccgtgggtg | aagagcggcctaagcactgcac | 1 | 601 | RsaI | 143, 126, 96, 95, 66, 41, 34 |
| 53-1 | ttgttcagtgtacggggctagc | gtgacgccattgcagttatcgcct | 6 | 549 | MspI | 389, 160 |
| 53-2 | ttctgcagtaagctatgagggcat | aaccactgtcgatttcggtgtt | 7 | 449 | MspI | 270, 179 |
| 56-1 | ctgggcactaggtcaaagcctgct | caaccacgcgtaaaagcactcat | 6 | 307 | AluI | 278, 29 |
| 58-1 | ggtagtaccccaccgtctgagg | agacgtgacattgccactgtca | 1 | 414 | MspI | 289, 125 |
| 58-2 | accagactccagagacaacacc | tcacctttgtcatcactggtcc | 1 | 264 | RsaI | 165, 62, 25, 12 |
| 59-1 | agacaccgttacatgagctgct | tcattctcggagtcggagtcag | 1 | 320 | AluI | 213, 91, 16 |
| 59-2 | tctaacgccatctgcagcaagg | acagtagtccactgacacgctg | 4 | 438 | HaeIII | 339, 99 |
| 66-1 | tgcggtagtatccttgggcagtg | tacaataagggctacacgccaa | 5 | 388 | RsaI | 135, 131, 122 |
| 68-1 | ggtactgcttggaacacgcctg | ggcccccagacatagggacctt | 6 | 368 | RsaI | 307, 57, 4 |
| 68-2 | gtcaaaaagacgcccctgcaccta | cacaccttagggtagggctacaa | 5 | 490 | HaeIII | 329, 161 |
| 73-1 | acaggctattagttgccaacgtc | ttcttaggtgtggcacttgtg | 6 | 222 | AluI | 115, 62, 45 |
| 73-2 | ggggtgggcaaaggtaggtagc | acaatccaggggcctctggtccga | 7 | 322 | RsaI | 189, 108, 25 |
| 82-1 | tgtccgtggacacctgcgacca | gtagttaaaggtgatgtggcaacc | 7 | 546 | RsaI | 275, 215, 56 |
| 82-2 | cccaaaaccaatacacgtgctgaa | aacatcctgttggtcgttgcca | 5 | 270 | HaeIII | 189, 81 |
| β-globin | gaagagccaaggacaggtac | caacttcatccacgttcacc | 7 | 268 |