Figure 6.

The miR-9 expression is not regulated by PPARδ in monocytes. (A) Human primary monocytes were seeded in 6-well plates (2×10⁶ cells/well) and treated with 100 nM PPARδ agonist (GW501516) for 2 h followed by isolation of RNA from the cells. To verify the potency of GW501516, mRNA expression of the PPARδ target gene, PLIN2, was analysed by real-time PCR. (B) The expression of miR-9 is shown from the same cells. (C) The expression of miR-9 in M1 and M2 macrophages, respectively, is shown after 4 h of treatment with GW501516. The experiment was repeated in monocytes from two individuals and in macrophages from two individuals. Representative results from these experiments are shown. White bars show results from no GW501516 treated cells and the results were set to one. PLIN2 expression was normalized to PPIA expression and miR-9 expression was normalized to U48 expression. Each bar represents the mean ± SD for 3 wells. ^*P<0.05.