Table 2.

Experimental design and results of the Box-Behnken design.

Run	A: K2HPO4	B: Yeast extract	C: MgSO4	D: NH4NO3	E: Dextrose	Chromium %
	(g L−1)	(g L−1)	(g L−1)	(g L−1)	(g L−1)	Experimental	Predicted
1	0.2	5.5	0.22	0.6	5	65.85	65.43
2	0.2	5.5	0.22	0.6	20	87.08	81.58
3	0.6	5.5	0.22	0.2	20	66.55	65.15
4	0.6	5.5	0.22	1	5	34.97	35.00
5	0.6	10	0.22	0.2	12.5	85.75	86.09
6	0.6	1	0.04	0.6	12.5	56.97	68.53
7	0.6	10	0.22	1	12.5	48.97	49.32
8	0.6	5.5	0.22	1	20	69.76	68.55
9	1	5.5	0.4	0.6	12.5	54.85	54.82
10	1	5.5	0.22	0.6	5	37.09	40.32
11	0.2	5.5	0.22	1	12.5	67.98	64.17
12	0.6	10	0.4	0.6	12.5	65.97	64.03
13	0.6	1	0.4	0.6	12.5	58.66	58.77
14	0.6	5.5	0.22	0.6	12.5	85.08	79.48
15	0.6	1	0.22	0.6	20	57.87	59.09
16	0.6	5.5	0.04	1	12.5	68.98	67.81
17	0.6	5.5	0.22	0.6	12.5	87.98	89.03
18	0.6	5.5	0.04	0.6	5	54.09	59.51
19	0.2	5.5	0.04	0.6	12.5	76.98	78.71
20	0.2	1	0.22	0.6	12.5	65.54	65.90
21	0.6	10	0.22	0.6	5	45.96	46.53
22	0.2	10	0.22	0.6	12.5	65.86	68.27
23	0.6	5.5	0.4	0.2	12.5	78.76	75.65
24	1	1	0.22	0.6	12.5	37.98	34.13
25	0.6	5.5	0.04	0.2	12.5	98.86	99.08
26	0.2	5.5	0.4	0.6	12.5	56.98	60.01
27	1	5.5	0.22	0.6	20	50.05	55.84
28	0.6	5.5	0.04	0.6	20	88.76	79.07
29	1	10	0.22	0.6	12.5	51.53	49.74
30	0.6	1	0.22	1	12.5	74.65	77.26
31	1	5.5	0.22	0.2	12.5	69.43	71.60
32	0.6	5.5	0.22	0.6	12.5	76.38	72.96
33	0.6	1	0.22	0.6	5	64.31	57.20
34	1	5.5	0.22	1	12.5	53.86	49.12
35	1	5.5	0.04	0.6	12.5	74.93	81.97
36	0.6	10	0.22	0.6	20	38.85	37.75
37	0.6	5.5	0.4	1	12.5	81.64	73.45
38	0.6	5.5	0.22	0.2	5	83.75	79.58
39	0.2	5.5	0.22	0.2	12.5	63.76	66.86
40	0.6	5.5	0.22	0.6	12.5	79.05	82.77
41	0.6	10	0.04	0.6	12.5	63.67	67.21
42	0.6	5.5	0.22	0.6	12.5	83.61	83.60
43	0.6	5.5	0.4	0.6	20	65.54	62.33
44	0.6	1	0.22	0.2	12.5	58.65	58.25
45	0.6	5.5	0.22	0.6	12.5	85.38	82.77
46	0.6	5.5	0.4	0.6	5	46.86	48.76