Table 3.

Protein spots undergoing significant changes (>2-fold) after storage at -20°C with respect to time zero

Spot	Protein name	Average ratio -20°C/time zero	Paired t-test
3	Serum albumin	0.20	0.047
4	Serum albumin	0.20	0.022
6	Alpha-1-antitrypsin	0.31	0.016
8	Fibrinogen beta chain	2.51	0.046
14	Serotransferrin	0.49	0.007
15	Serotransferrin	0.29	0.016
16	Apolipoprotein A-I	0.20	0.039