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. 2013 Apr 10;11:12. doi: 10.1186/1477-3155-11-12

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Copyright © 2013 Moolman et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Schematic of the fabrication of multiple structures in PDMS with sub-micron sized growth channels. (Step 1) The wafer is cleaned with HNO₃. (Step 2–3) A 1:5 ratio PDMS is poured on the wafer, cured, and carefully peeled off. (Step 4) A silanization step is performed on the resulting PDMS mold. (Step 5) A 1:10 ratio PDMS is poured on the PDMS mold and cured. (Steps 6) The two PDMS layers are carefully separated from one other. (Step 7) A single structure is cut out, and an inlet and outlet port are punched. (Step 8) PDMS and a clean cover glass are bound together using O₂ plasma.