Figure 5. Recruitment and/or activation of DCs and B cells in cervical and mesenteric lymph nodes as well as blood after booster oral immunization.
BALB/c mice were orally immunized with 1×107 FFU of rRABVs or DMEM and booster oral immunization was carried out at day 21 after the primary immunization by immunizing with the same dose. Then the cervical and mesenteric lymph nodes as well as the blood were collected on 3, 6 and 9 days after boost immunization. Single cell suspensions were prepared and stained with antibodies for DCs (CD11c+ and CD86+) (A, B and C) and B cells (CD19+ and CD40+) (D, E and F). Asterisks indicate significant differences between the experimental groups as analyzed by one-way ANOVA (*,P<0.05;**,P<0.01;***,P<0.001).