Skip to main content
. 2013 May 20;8(5):e63712. doi: 10.1371/journal.pone.0063712

Figure 7. Activation of caspase 3/7 and cleavage of PARP following depletion of TTK in triple-negative breast cancer cells.

Figure 7

(A to D) Increased caspase 3/7 activity in TTK-depleted cells. At the indicated time we measured caspase 3/7 activity in TNBC cells, MDA-MB-468 (A), HCC70 (B) and MDA-MB-231 (C), and normal MCF10A cells (D) following siRNA-transfection performed as in Figure 4. Each value in the histograms corresponds to the fold change in caspase 3/7 activity and represents the mean of three independent experiments. The error bars represent the standard deviation of the mean and the asterisks, the p values from Student’s t test (*p<0.05; **p<0.01; ***p<0.001). (E to H) Increased cleavage of caspase 7 and PARP in TTK-depleted cells. We transfected MDA-MB-468 (E), HCC70 (F), MDA-MB-231 (G) and MCF10A (H) cells with the indicated siRNAs. At the indicated time points, we harvested the cells and performed immunoblotting with antibodies against (i) cleaved PARP, which recognizes both uncleaved (PARP) and cleaved (c-PARP) forms, (ii) cleaved caspase 7 (c-casp7) and (iii) phosphorylated H2AX (γH2AX). We used an anti-TTK antibody to confirm TTK depletion and β-actin as a loading control.