Skip to main content
. 2013 May 7;13:207. doi: 10.1186/1471-2334-13-207

Table 3.

IC50 values for TgCPB and TgCPL peptide binding to MHC class II molecules obtained using the immune epitope database

MHC II Allele1 Start-Stop2 Sequence3 Percentile Rank4
TgCPB
 
 
 
HLA-DRB1*01:01
10–24
LGTPLPFAALAAILL
0.01
HLA-DRB1*01:01
71–85
ESLFDSIVAEQVVAT
6.21
HLA-DRB1*01:01
228–242
LRFRYLSLKDAKKLM
4.99
HLA-DRB1*01:01
320–334
SQGKRLMPLSAQHTT
2.74
HLA-DRB1*01:01
470–484
SGVYKHVSGLPVGGH
1.58
H2-IAb
8–22
RVLGTPLPFAALAAI
3.83
H2-IAb
59–73
DVLNAFVSPESVESL
3.51
H2-IAb
187–201
VFWESRPASSNAAVA
0.91
H2-IAb
300–314
CGSCWAFASTEAFND
4.46
H2-IAb
549–563
LPGQRAAGARAGAHA
3.19
H2-IAd
15–29
PFAALAAILLLGCMY
1.88
H2-IAd
191–205
SRPASSNAAVALIKK
4.34
H2-IAd
227–241
SLRFRYLSLKDAKKL
1.21
H2-IAd
320–334
SQGKRLMPLSAQHTT
3.19
H2-IAd
428–442
QDTHKATSAYSLRSR
2.52
H2-IEd
182–196
ETGEDVFWESRPASS
17.89
H2-IEd
226–240
VSLRFRYLSLKDAKK
13.43
H2-IEd
351–365
QPGMAWRWFERKGVV
4.77
H2-IEd
399–413
DATLVPRKTPKCRKD
16.82
H2-IEd
432–446
KATSAYSLRSRDDVK
9.89
TgCPL
 
 
 
HLA-DRB1*01:01
60–74
RAWIALVAAAVSLLV
0.04
HLA-DRB1*01:01
46–60
YFWKKFLRQRNFTAR
6.00
HLA-DRB1*01:01
117–131
FSSFQAMYAKSYATE
2.74
HLA-DRB1*01:01
136–150
RRYAIFKNNLVYIHT
3.24
HLA-DRB1*01:01
178–192
LGFKKSRNLKSHHLG
5.20
H2-IAb
27–41
RRGVRAGRPSPPFVV
1.49
H2-IAb
59–73
ARAWIALVAAAVSLL
3.37
H2-IAb
85–99
EDDRAVFPPSPVEDH
3.42
H2-IAb
116–130
AFSSFQAMYAKSYAT
5.68
H2-IAb
226–240
CGSCWAFSTTGALEG
2.49
H2-IAd
22–36
GELHQRRGVRAGRPS
6.17
H2-IAd
57–71
FTARAWIALVAAAVS
0.14
H2-IAd
117–131
FSSFQAMYAKSYATE
2.63
H2-IAd
318–332
DVPRRSEAAMKAALA
0.01
H2-IAd
395–409
RDGYMYMAMHKGEEG
1.82
H2-IEd
42–56
TTRTYFWKKFLRQRN
7.05
H2-IEd
174–188
RRKYLGFKKSRNLKS
13.03
H2-IEd
202–216
PSELPAGVDWRSRGC
28.28
H2-IEd
309–323
KVVKILGFKDVPRRS
21.70
H2-IEd 376–390 KESKKDFWIMKNSWG 24.70

1 H2-IAb, H2-IAd and H2-IEd alleles are mouse MHC class II molecules; the HLA-DRB1*01:01 allele is a human MHC class II molecule.

2 We chose 15 amino acids for analysis each time.

3 TgCPB or TgCPL sequences.

4 Low percentile = high binding.